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Antibody Dilution Calculator (1:N)
Enter the dilution ratio and the working volume you need, and get the stock volume in μL and the diluent to top up in mL. For primary and secondary antibodies, enzymes, dyes and any reagent diluted 1:N.
Inputs
mL
Results
- Stock to take
- 20 μL
- Diluent (top up)
- 9.98 mL
Formula
Stock (μL) = Total (mL) × 1000 ÷ N
You got this step right, but a whole experiment has dozens of linked parameters. Change one dilution factor and everything downstream has to be recalculated. In Vara the parameters of your whole experiment are connected, so changing one recalculates the rest automatically. Manage 3 experiments for free.
FAQ
What does a 1:500 dilution mean and how do I make it?+
1:500 means 1 part stock brought to a total of 500 parts with diluent. For 10 mL of working solution, take 10 mL × 1000 ÷ 500 = 20 μL of stock and top up with diluent to 10 mL. Top up to the final volume; do not simply add 10 mL of diluent.
How do I convert between μL and mL?+
1 mL = 1000 μL. Working volumes are usually given in mL while the stock volume is often only tens of μL, so the formula multiplies by 1000 to convert mL to μL. This calculator switches to mL automatically above 1000 μL.
How long can a diluted antibody be kept?+
Prepare it fresh when you can. Once diluted, the antibody is at a low concentration and more prone to sticking to tube walls, protease degradation and microbial growth, so the signal fades over time. If you must store it, follow the datasheet: usually short-term at 4 °C with a preservative such as sodium azide, and avoid repeated freeze-thaw.